Duck hepatitis A virus (DHAV) genotype definition: Comment on the article by Cha et al.

文献类型： 外文期刊

第一作者： Wen, Huiqiang

作者： Wen, Huiqiang;Han, Lingxia;Zhang, Xiaona;Lian, Chuanjiang;Zhao, Lili;Si, Changde;Chen, Hongyan

作者机构：

关键词： Duck hepatitis A virus;Genotype definition;Pairwise sequence comparisons (PASC)

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2014 年 170 卷 3-4 期

页码：

收录情况： SCI

摘要： Duck hepatitis A virus (DHAV) is genetically divided into three different genotypes: the original type DHAV-1, a type recently isolated in Taiwan (DHAV-2), and a recently described type isolated in South Korea and China (DHAV-3). Recently, Cha et al. (2013) concluded that the existence that both DHAV-1 and DHAV-2 had been classified into one branch, with DHAV genotype 3 (DHAV-3) in another, and that the phylogenetic distance unit showed was 0.5, a tremendous value. However, there might be some concerns on the methodology application to define the genotypes of DHAV. Based on 110 genomic and 100 amino acid sequences of DHAV which included all the sequences from Cha et al. (2013) respectively, phylogenetic analysis in the present study showed a distinct and proposed DHAV genotype definition, that both DHAV-2 and DHAV-3 were clustered in one branch while DHAV-1 in another branch only, and that the phylogenetic distance unit of 0.02 was confirmed, which was much smaller than the value 0.5. Taking into account the genotype definition of DHAV, we also conducted the pairwise sequence comparisons (PASC) analysis of 110 genomic sequences, and proposed that the distance genotype definition threshold was 0.045. (C) 2014 Elsevier B.V. All rights reserved.

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