Individual-breed assignment analysis in swine populations by using microsatellite markers

文献类型： 外文期刊

第一作者： Fan, B

作者： Fan, B;Chen, YZ;Moran, C;Zhao, SH;Liu, B;Yu, M;Zhu, MJ;Xiong, TA;Lil, K

作者机构：

关键词： individual-breed assignment;assignment accuracy;microsatellite;pig

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2005 年 18 卷 11 期

页码：

收录情况： SCI

摘要： Individual-breed assignments were implemented in six swine populations using twenty six microsatellites recommended by the Food and Agriculture Organization and the International Society for Animal Genetics (FAO-ISAG). Most microsatellites exhibited high polymorphisms as shown by the number of alleles and the polymorphism information content. The assignment accuracy per locus obtained by using the Bayesian method ranged from 33.33% (CGA) to 68.47% (S0068), and the accumulated assignment accuracy of the top ten loci combination added up to 96.40%. The assignment power of microsatellites based on the Bayesian method had positive correlations with the number of alleles and the gene differential coefficient (G(st)) per locus, while it has no relationship to genetic heterozygosity, polymorphism information content per locus and the exclusion probabilities under case II and case III. The percentage of corrected assignment was highest for the Bayesian method, followed by the gene frequency and distance-based methods. The assignment efficiency of microsatellites rose with increase in the number of loci used, and it can reach 98% when using a ten-locus combination. This indicated that such a set of ten microsatellites is sufficient for breed verification purposes.

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