文献类型： 外文期刊
第一作者： Song, Zhiqiang
作者： Song, Zhiqiang;Li, Yuan;Liu, Yang;Xin, Jiuqing;Zou, Xiaohui;Sun, Wenjing
作者机构：
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2012 年 7 卷 6 期
页码：
收录情况： SCI
摘要： Mycoplasma bovis is the causative agent of Mycoplasma bovis-associated disease (MbAD). Although the mechanisms underlying M. bovis adherence to host cells is not clear, recent studies have shown that the cell surface protein alpha-enolase facilitates bacterial invasion and dissemination in the infected host. In this study, we cloned, expressed and purified recombinant M. bovis alpha-enolase and induced polyclonal anti-alpha-enolase antibodies in rabbits. M. bovis alpha-enolase was detected in the cytoplasmic and membrane protein fractions by these antibodies. Triple immunofluorescence labeling combined with confocal laser scanning microscopy (CLSM) revealed that the plasminogen (Plg) enhanced the adherence of M. bovis to embryonic bovine lung (EBL) cells; the values obtained for adherence and inhibition are consistent with this finding. Interestingly, we found that trace amounts of trypsin acted as a more effective enhancer of cell adherence than Plg. Hence, our data indicate that surface-associated M. bovis alpha-enolase is an adhesion-related factor of M. bovis that contributes to adherence by binding Plg.
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