Effects of Peroxisome Proliferator-Activated Receptor ? on Modulating Angiopoietin-Like Protein 4 Synthesis in Caco-2 Cells Exposed to Clostridium butyricum

文献类型： 外文期刊

第一作者： Zhao, X.

作者： Zhao, X.;Huang, H. S.;Shi, S. R.

作者机构：

关键词： Clostridium butyricum; peroxisome proliferator activated receptor gamma; angiopoietin-like protein 4; Caco-2 cell

期刊名称：MOLECULAR BIOLOGY （ 影响因子：1.2； 五年影响因子：1.2 ）

ISSN： 0026-8933

年卷期： 2023 年 57 卷 3 期

页码：

收录情况： SCI

摘要： Angiopoietin-like protein 4 (ANGPTL4) is considered to be one of the important circulating mediators linking intestinal microorganisms and host lipid metabolism. The objective of this study was to assess the effects of peroxisome proliferator-activated receptor gamma (PPAR gamma) on modulating ANGPTL4 synthesis in Caco-2 cells exposed to Clostridium butyricum. The viability of Caco-2 cells and the expression of PPAR gamma and ANGPTL4 in Caco-2 cells were detected after the Caco-2 cells were co-cultured with C. butyricum at the concentration of 1 x 10(6), 1 x 10(7) and 1 x 10(8) CFU/mL. The results showed that cell viability was enhanced by C. butyricum. Besides, PPAR gamma and ANGPTL4 expression and secretion in Caco-2 cells was significantly increased by 1 x 10(7) and 1 x 10(8) CFU/mL of C. butyricum. Furthermore, the effects of PPAR gamma on modulating ANGPTL4 synthesis in Caco-2 cells regulated by 1 x 10(8) CFU/mL of C. butyricum was also be expounded in PPAR gamma activation/inhibition model based on Caco-2 cells and via ChIP technique. It was found that C. butyricum promoted the binding of PPAR gamma to the PPAR binding site (chr19: 8362157-8362357, located upstream of the transcriptional start site of angptl4) of the angptl4 gene in Caco-2 cells. However, the PPAR gamma was not the only way for C. butyricum to stimulate ANGPTL4 production. Taken together, PPAR gamma played a role in the regulation of ANGPTL4 synthesis by C. butyricum in Caco-2 cells.

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