农科机构知识库联盟

SPIKE PROTEIN REGION (aa 636-789) OF PORCINE EPIDEMIC DIARRHEA VIRUS IS ESSENTIAL FOR INDUCTION OF NEUTRALIZING ANTIBODIES

文献类型：外文期刊

第一作者： Sun, D. B.

作者： Sun, D. B.;Feng, L.;Shi, H. Y.;Chen, J. F.;Liu, S. W.;Chen, H. Y.;Wang, Y. F.

作者机构：

关键词： epitope;Porcine epidemic diarrhea virus;spike protein;virus neutralization

期刊名称：ACTA VIROLOGICA （影响因子：1.162；五年影响因子：1.255 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To analyze antigenic structure of the spike (S) protein of Porcine epidemic diarrhea virus (PEDV), the gene encoding its major immunodominant region S1 was amplified by PCR. We prepared four truncated S1 proteins spanning the entire S1 domain fused to GST protein. To identify the most important antigenic region of S1, the truncated S1-GST fusion proteins were examined for their ability to react with immune serum against PEDV and to elicit the formation of neutralization antibodies in immunized animals. We found that the region of S1 signed as S1D (aa 636-789) was able to react with PEDV antiserum and to elicit formation of neutralization antibodies in mice. Moreover, the immune serum against S1D showed the binding ability to the native S protein of PEDV.

分类号： R373

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