A polyketide synthase from Verticillium dahliae modulates melanin biosynthesis and hyphal growth to promote virulence

文献类型： 外文期刊

第一作者： Li, Huan

作者： Li, Huan;Geng, Qi;Sheng, Ruo-Cheng;Chen, Feng-Mao;Li, Huan;Wang, Dan;Zhang, Dan-Dan;Geng, Qi;Li, Jun-Jiao;Sheng, Ruo-Cheng;Xue, Hui-Shan;Zhu, He;Kong, Zhi-Qiang;Dai, Xiao-Feng;Chen, Jie-Yin;Zhu, He;Klosterman, Steven J.;Subbarao, Krishna, V

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关键词： Verticillium dahliae; Polyketide synthase; Melanin; Microsclerotia; Hyphal growth; Virulence

期刊名称：BMC BIOLOGY （ 影响因子：7.364； 五年影响因子：8.641 ）

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年卷期： 2022 年 20 卷 1 期

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收录情况： SCI

摘要： Background During the disease cycle, plant pathogenic fungi exhibit a morphological transition between hyphal growth (the phase of active infection) and the production of long-term survival structures that remain dormant during "overwintering." Verticillium dahliae is a major plant pathogen that produces heavily melanized microsclerotia (MS) that survive in the soil for 14 or more years. These MS are multicellular structures produced during the necrotrophic phase of the disease cycle. Polyketide synthases (PKSs) are responsible for catalyzing production of many secondary metabolites including melanin. While MS contribute to long-term survival, hyphal growth is key for infection and virulence, but the signaling mechanisms by which the pathogen maintains hyphal growth are unclear. Results We analyzed the VdPKSs that contain at least one conserved domain potentially involved in secondary metabolism (SM), and screened the effect of VdPKS deletions in the virulent strain AT13. Among the five VdPKSs whose deletion affected virulence on cotton, we found that VdPKS9 acted epistatically to the VdPKS1-associated melanin pathway to promote hyphal growth. The decreased hyphal growth in VdPKS9 mutants was accompanied by the up-regulation of melanin biosynthesis and MS formation. Overexpression of VdPKS9 transformed melanized hyphal-type (MH-type) into the albinistic hyaline hyphal-type (AH-type), and VdPKS9 was upregulated in the AH-type population, which also exhibited higher virulence than the MH-type. Conclusions We show that VdPKS9 is a powerful negative regulator of both melanin biosynthesis and MS formation in V. dahliae. These findings provide insight into the mechanism of how plant pathogens promote their virulence by the maintenance of vegetative hyphal growth during infection and colonization of plant hosts, and may provide novel targets for the control of melanin-producing filamentous fungi.

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[1]A polyketide synthase from Verticillium dahliae modulates melanin biosynthesis and hyphal growth to promote virulence. Li, Huan,Geng, Qi,Sheng, Ruo-Cheng,Chen, Feng-Mao,Li, Huan,Wang, Dan,Zhang, Dan-Dan,Geng, Qi,Li, Jun-Jiao,Sheng, Ruo-Cheng,Xue, Hui-Shan,Zhu, He,Kong, Zhi-Qiang,Dai, Xiao-Feng,Chen, Jie-Yin,Zhu, He,Klosterman, Steven J.,Subbarao, Krishna, V. 2022

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