Abundance for subgroups of denitrifiers in soil aggregates asscociates with denitrifying enzyme activities under different fertilization regimes

文献类型： 外文期刊

第一作者： Luo, Xuesong

作者： Luo, Xuesong;Zeng, Luyang;Wang, Li;Qian, Hang;Hou, Chunli;Huang, Qiaoyun;Chen, Wenli;Luo, Xuesong;Zeng, Luyang;Wang, Li;Qian, Hang;Hou, Chunli;Huang, Qiaoyun;Wen, Shilin;Wang, Boren

作者机构：

关键词： nirK; nirS; nosZ; Soil aggregate size fractions; Denitrification

期刊名称：APPLIED SOIL ECOLOGY （ 影响因子：4.046； 五年影响因子：4.884 ）

ISSN： 0929-1393

年卷期： 2021 年 166 卷

页码：

收录情况： SCI

摘要： This study coupled a measurement of denitrifier gene abundances at the phylogroup level with denitrifying enzyme activities to show how long-term fertilization shaped the community of denitrifiers inhabiting the soil aggregates of various sizes. Three groups of nirK, including clade I (nirK-I), clade II (nirK-II), and group IIa (nirKIII), clade I nirS (nirS-I), and two clades of nosZ (nosZ-I and nosZ-II) were detectable. Most of these genes were increased by the fertilization with swine manure and subsequently diverged across aggregate size fractions. Both nirK-I and nirK-II were mainly driven by the available phosphorus (AP) and total nitrogen (TN), and nirK-III (group IIa) by pH. NirS-I was best predicted by total phosphorus and TN. For both nosZ-I and nosZ-II, TN and total carbon are the best predictors. Group IIa nirK and nirS-I nitrite reducers possibly possessed an advantage in the soil aggregates with a lower nutrient level than the other nitrite reducers, and nosZ-II N2O reducers were more competitive at a condition with a lower pH. The gross N2O production and the net N2O production were both stimulated by the fertilization with swine manure, and the former also significantly differed across different soil aggregate size fractions. Structure equation modeling analysis indicated that the Gross N2O production was dominantly explained by the nirK-I nitrite-reducers and total nitrogen, whereas the net N2O production by the total phosphorus and the sum of nosZ-II and nosZ-I N2O reducers, which were also associated with the Gross N2O production. Our results also highlighted that microaggregates with manure fertilization are likely hot spots for denitrifiers in the soil.

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