Identification of long non-coding RNAs in response to microsporidia infection in Silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Pu, Shiqi

作者： Pu, Shiqi;Fang, Yujia;Yang, Yuhang;Qu, Qingsheng;Liu, Mengjin;Tang, Xudong;Shen, Zhongyuan;Qian, Ping;Lian, Jialin;Qian, Ping;Tang, Xudong;Shen, Zhongyuan;Qian, Ping

作者机构：

关键词： Bombyx mori; lncRNA; Nosema bombycis; lncRNA-miRNA-mRNA

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2024 年 117 卷 3 期

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收录情况： SCI

摘要： Microsporidia Nosema bombycis (Nb) is a cellular parasite responsible for p & eacute;brine disease in silkworms, significantly impacting the sericulture industry. Long non-coding RNAs (lncRNAs), which are RNA fragments longer than 200 nucleotides, are pivotal in a range of cellular and physiological functions. However, the potential role of silkworm lncRNAs in response to Nb infection remains unknown. This study conducted transcriptome sequencing on both larvae and Nb-infected midguts of silkworms, identifying 1,440 lncRNAs across all examined midgut samples. Within the Nb-infected group, 42 differentially expressed lncRNAs (DElncRNAs) and 305 differentially expressed mRNAs (DEmRNAs) were detected. Functional annotation and pathway analysis showed that these DEmRNAs are mostly involved in metabolism, apoptosis, autophagy, and other key pathways. The co-expression network of DEmRNAs and DElncRNAs illustrates that 1 gene could be regulated by multiple lncRNAs and 1 lncRNA may target multiple genes, indicating that the regulation of lncRNA is intricate and networked. In addition, the DElncRNA-miRNA-mRNA network showed that some DElncRNAs may be involved in the immune response and metabolism through miRNA. Notably, the study observed an increase in lncRNA MSTRG857.1 following Nb infection, which may promote Nb proliferation. These findings offer insights into the complex interplay between insects and microsporidia.

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