XBP1s gene of endoplasmic reticulum stress enhances proliferation and osteogenesis of human periodontal ligament cells

文献类型： 外文期刊

第一作者： Cui, Ziwei

作者： Cui, Ziwei;Qin, Ruoshan;Feng, Jianbao;Liu, Yuan;Zhou, Xiongtao;He, Xiangyi;Li, Yanmin;Zhang, Zhidong;He, Xiangyi;Qin, Xiaodong;Cui, Ziwei

作者机构：

关键词： Endoplasmic reticulum stress; Autophagy; Osteogenesis; Human periodontal ligament cells; Apoptosis

期刊名称：TISSUE & CELL （ 影响因子：2.6； 五年影响因子：2.5 ）

ISSN： 0040-8166

年卷期： 2023 年 83 卷

页码：

收录情况： SCI

摘要： Background: The endoplasmic reticulum stress (ERS) pathway, inositol-requiring enzyme-1 alpha-X-box binding protein-1 (IRE1 & alpha;-XBP1), has been considered as a critical factor of human periodontal ligament cells (hPDLCs) in proliferation and osteogenesis. This study aimed to explore the effect and mechanism of XBP1s, which was cleaved by IRE1 & alpha; on the proliferation and osteogenesis of hPDLCs. Methods: ERS model was induced by tunicamycin (TM); cell proliferation was assessed by CCK-8 assay; pLVXXBP1s-hPDLCs cell line was established by lentivirus infaction; expression of ERS-related protein including eIF2 & alpha;, GRP78, ATF4 and XBP1s, autophagy-related P62 and LC3, and apoptosis-related Bcl-2 and Caspase-3 were detected by Western Blot; expression of osteogenic genes was detected by RT-qPCR, and senescence of hPDLCs was explored by & beta;-galactosidase staining. Furthermore, the interaction between XBP1s and human bone morphogenetic protein 2 (BMP2) was examined by immunofluorescence antibody test (IFAT).Results: The results showed an increase in proliferation of hPDLCs from 0 to 24 h when ERS was induced by TM treatment (P < 0.05). XBP1s overexpression induced hPDLCs proliferation, upgraded autophagy and degraded apoptosis significantly (P < 0.05). In pLVX-XBP1s-hPDLCs, the ratio of senescent cells was markedly decreased after several passages (P < 0.05); After infection with pLVX-BMP2 lentiviral supernatant, IFAT result showed that XBP1s and BMP2 well co-located in the cytoplasm of pLVX-XBP1s-hPDLCs and PERK-ATF4 ERS branch was activated, meanwhile, there were obviously more mineralized nodules and mRNA expression of osteogenesisrelated genes was continually up-regulated (P < 0.05).Conclusions: XBP1s promotes the proliferation via regulating the autophagy and apoptosis, and enhances expression of osteogenic genes in hPDLCs. The mechanisms in this regard need exploring further for periodontal tissue regeneration, functionalization and clinical applications.

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