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A tonoplast-localized TPK-type K plus transporter (TPKa) regulates potassium accumulation in tobacco

文献类型：外文期刊

第一作者： Gao, Yulong

作者： Gao, Yulong;Zhao, Lu;Wang, Bingwu;Song, Zhongbang;Jiao, Fangchan;Wu, Xingfu;Feng, Zhiyu;Chen, Xuejun;Li, Yongping;Gao, Lifeng

作者机构：

关键词： Tobacco; NtTPKa; K plus concentration in tobacco; K plus transporter

期刊名称：GENE （影响因子：2.6；五年影响因子：2.7 ）

ISSN： 0378-1119

年卷期： 2024 年 926 卷

页码：

收录情况： SCI

摘要： Potassium ion (K + ) is one of the most essential nutrients for the growth and development of tobacco ( Nicotiana tabacum L.), however, the molecular regulation of K + concentration in tobacco remains unclear. In this study, a two -pore K (TPK) channel gene NtTPKa was cloned from tobacco, and NtTPKa protein contains the unique K + selection motif GYGD and its transmembrane region primarily locates in the tonoplast membrane. The expression of NtTPKa gene was significantly increased under low -potassium stress conditions. The concentrations of K + in tobacco were significantly increased in the NtTPKa RNA interference lines and CRISPR/Cas9 knockout mutants. In addition, the transport of K + by NtTPKa was validated using patch clamp technique, and the results showed that NtTPKa channel protein exclusively transported K + in a concentration -dependent manner. Together, our results strongly suggested that NtTPKa is a key gene in maintaining K + homeostasis in tobacco, and it could provide a new genetic resource for increasing the concentration of K + in tobacco.

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