Functional analysis of AgJHAMT gene related to developmental period in Aphis gossypii Glover

文献类型： 外文期刊

第一作者： Zhang, Lianjun

作者： Zhang, Lianjun;Li, Yuan;Xu, Xinhui;Feng, Mengmeng;Turak, Rukiya;Liu, Xiaoning;Pan, Hongsheng

作者机构：

关键词： Aphis gossypii; gene function; juvenile hormone acid methyltransferase; methoprene rescue

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.6； 五年影响因子：1.7 ）

ISSN： 0007-4853

年卷期： 2024 年 114 卷 5 期

页码：

收录情况： SCI

摘要： Aphis gossypii is one of the most economically important agricultural pests that cause serious crop losses worldwide, and the indiscriminate chemical application causes resistance development in A. gossypii, a major obstacle to successful control. In this study, we selected the up-regulated expression gene AgJHAMT, which was enriched into juvenile hormone pathway though transcriptome sequencing analysis of the cotton aphids that fed on transgenic cotton lines expressing dsAgCYP6CY3 (the TG cotton). The AgJHAMT gene was overexpressed in cotton aphids which fed on the TG cotton, and its expression profile during the nymphs was clarified. Then, silencing AgJHAMT could advance the developmental period of cotton aphids by 0.5 days compared with control groups. The T and t values of cotton aphids in the dsJHAMT treatment group (6.88 +/- 0.15, 1.65 +/- 0.06) were significantly shorter than that of the sprayed H2O control group (7.6 +/- 0.14, 1.97 +/- 0.09) (P < 0.05), respectively. The fast growth caused by AgJHAMT silencing was rescued by applying the JH analogue, methoprene. Overall, these findings clarified the function of AgJHAMT in the developmental period of A. gossypii. This study contributes to further clarify the molecular mechanisms of delaying the growth and development of cotton aphids by the transgenic cotton lines expressing dsAgCYP6CY3.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序