Characterization of glucosinolates in 80 broccoli genotypes and different organs using UHPLC-Triple-TOF-MS method

文献类型： 外文期刊

第一作者： Li, Zhansheng

作者： Li, Zhansheng;Zheng, Shuning;Liu, Yumei;Fang, Zhiyuan;Yang, Limei;Zhuang, Mu;Zhang, Yangyong;Lv, Honghao;Wang, Yong;Xu, Donghui;Li, Zhansheng;Zheng, Shuning;Liu, Yumei;Fang, Zhiyuan;Yang, Limei;Zhuang, Mu;Zhang, Yangyong;Lv, Honghao;Wang, Yong;Xu, Donghui;Li, Zhansheng;Zheng, Shuning;Xu, Donghui

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关键词： Broccoli; Genotype; Organs; UHPLC-Triple-TOF-MS; Glucosinolate

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 334 卷

页码：

收录情况： SCI

摘要： We aimed to characterize and quantify glucosinolate compounds and contents in broccoli, and a total of 80 genotypes and eight developmental organs were analyzed with UHPLC-Triple-TOF-MS. The method was validated in terms of performance, and the coefficients of determination (R-2) were 0.97 and 0.99 for glucoraphanin and gluconapin, respectively. In 80 genotypes, twelve glucosinolates were found in broccoli florets ranging from 0.467 to 57.156 mu mol/g DW, with the highest glucosinolate content being approximately 122-fold higher than the lowest value. The principal component of glucobrassicin, neoglucobrassicin and glucoraphanin explained 60.53% of the total variance. There were positive correlations among hydroxyglucobrassicin, methoxyglucobrassicin, glucobrassicin, glucoerucin, gluconasturtiin, glucoraphanin, and glucotropaeolin (P < 0.05). The root contained 43% of total glucosinolates in 80 genotypes, and glucoraphanin represented 29% of the total glucosinolate content in different organs. The mutant broccoli genotypes were found by analysis of gluconapin contents in different organs.

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