Investigating of type IV pili to the pathogenicity of Aeromonas schubertii

文献类型： 外文期刊

第一作者： Cao, Jizhen

作者： Cao, Jizhen;Wang, Qing;Wang, Linchuan;Cao, Jizhen;Liu, Chun;Wang, Qing;Zhang, Defeng;Chang, Ouqin;Wang, Yingying;Shi, Cunbin;Liu, Chun

作者机构：

关键词： Aeromonas schubertii; Type IV pili; Adhesion; Pathogenicity

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2021 年 530 卷

页码：

收录情况： SCI

摘要： Aeromonas schubertii is associated with multi-organ necrosis disease that frequently infects cultured snakehead fish. We examined whether type IV pili were important in the pathogenicity of A. schubertii. A Tap pubis mutant (Delta pili) was constructed by insertional mutagenesis of the tapM/N/O/P/Q gene cluster. Compared with the wild-type (WT) strain, the proliferation was not changed, but the sensitivity to oxidative stress and low pH were different. The mutant displayed increased survival within macrophages compared with the WT strain. In contrast, cell and tissue adhesion of the mutant was significantly lower than the WT strain. Fish challenged by immersion resulted in reduced cumulative mortality of the mutant compared with the WT strain although intraperitoneal infection was identical for both strains. Expression of IFN-gamma, IL-1, TCR, CC22, CD4 and CD40 in infected fish were similar between the two bacterial strains. Together these data indicated that tapM/N/O/P/Q is necessary for Tap pilus biogenesis in A. schubertii and adhesion is a key function for pathogenicity that is mediated by Tap pili.

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