Comparative proteomic analysis of hulless barley cultivars (Hordeum vulgare L.) differing distinctly in beta-glucan content

文献类型： 外文期刊

第一作者： Zhang, Guoqiang

作者： Zhang, Guoqiang;Yang, Kun;Xue, Wenhua;Zhang, Guoqiang;Zeng, Xingquan;Xu, Qijun;Wang, Yulin;Yuan, Hongjun;Nyima, Tashi;Yang, Kun;Zhang, Yuhong

作者机构：

关键词： Hulless barley; beta-glucan; Comparative proteomics; Parallel reaction monitoring (PRM); Candidate proteins

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）

ISSN： 0023-6438

年卷期： 2020 年 133 卷

页码：

收录情况： SCI

摘要： The hulless barley planted in Tibetan area, China (also called highland barley) is well known for their high beta-glucan content. This study investigated the proteomics of three highland barley cultivars differing in grain beta-glucan content (high, BH; middle, BM and low, BL) to identify the proteins involved in beta-glucan synthesis and accumulation and understand the mechanisms underlying the difference of beta-glucan content among these cultivars. Totally 38, 167 and 177 differentially expressed proteins (DEPs) were detected from BH VS BM, BH VS BL and BM VS BL comparisons, respectively. These DEPs are mainly enriched in the categories related to beta-glucan biosynthesis, carbohydrate metabolism, glucose and sucrose dissimilation. Many glycosidases and glycosyltransferases proteins involved in polysaccharide catabolism were significantly down-regulated in BH and BM cultivars in comparison with BL. The expression of some glycosidases proteins was further confirmed by Parallel Reaction Monitoring (PRM)-MS analysis. These results provide new insights into the proteins that might influence beta-glucan levels in highland barley cultivars, which could be helpful for genetic improvement of beta-glucan content in barley.

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