Knockout of SlDCL2b attenuates the resistance of tomato to potato spindle tuber viroid infection

文献类型： 外文期刊

第一作者： Zhang, Yuhong

作者： Zhang, Yuhong;Tian, Xiaxia;Xu, Huiyuan;Zhan, Binhui;Li, Shifang;Zhang, Zhixiang;Tian, Xiaxia;Zhou, Changyong

作者机构：

关键词： dicer proteins; microRNA; RNA silencing; siRNA; tomato; viroid

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.9 ）

ISSN： 1464-6722

年卷期： 2024 年 25 卷 3 期

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收录情况： SCI

摘要： RNA interference, or RNA silencing, is an important defence mechanism against viroid infection in plants. Plants encode multiple DICER-LIKE (DCL) proteins that are key components of the RNA silencing pathway. However, the roles of different DCLs in defence responses against viroid infection remain unclear. Here, we determined the function of tomato DCL2b (SlDCL2b) in defence responses against potato spindle tuber viroid (PSTVd) infection using SlDCL2b loss-of-function tomato mutant plants. Compared with wild-type plants, mutant plants were more susceptible to PSTVd infection, developing more severe symptoms earlier and accumulating higher levels of PSTVd RNAs. Moreover, we verified the feedback mechanism for the regulation of SlDCL2b expression by miR6026. Functional blocking of tomato miR6026, by expressing its target mimics, can enhance resistance to PSTVd infection in tomato plants. These findings deepen the current understanding of RNAi-based resistance against viroid infection and provide a potentially new strategy for viroid control.

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