文献类型： 外文期刊

第一作者： Li, Yu Qin

作者： Li, Yu Qin;Gu, Chun Tao;Tian, Wen Li

作者机构：

关键词： Weissella; novel species; yogurt; lactic acid bacteria

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 4 期

页码：

收录情况： SCI

摘要： Three Gram-stain-positive bacterial strains, designated X0750(T), X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 165 rRNA gene and three housekeeping genes (pheS, rpoA and recA), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), in silico DNA-DNA hybridization (isDDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750(T) was phylogenetically related to the type strains of Weissella hellenica, Weissella bombi, Weissella paramesenteroides. Weissella jogaejeotgali, Weissella thailandensis, Weissella oryzae, Weissella cibaria and Weissella confusa, having 94.4-100% 165 rRNA gene sequence similarities, 76.7-90.0%pheS gene sequence similarities. 88.9-99.4% rpoA gene sequence similarities and 77.6-92.8% recA gene sequence similarities, respectively. ANI, isDDH and AAI values between strain X0750(T) and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8% respectively, confirming that strain X0750(T) represents a novel species within the genus Weissella. Based upon the data obtained in the present study, a novel species, Weissella sagaensis sp. nov.. is proposed and the type strain is X0750(T)(=NCIMB 15192(T) =CCM 8924(T) =LMG 31184(T) =CCTCC AB 2018403(T)).

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