文献类型： 外文期刊

第一作者： Zhang, Hong Xia

作者： Zhang, Hong Xia;Gu, Chun Tao;Tian, Wen Li

作者机构：

关键词： genome; Lactococcus garvieae subsp. bovis; Lactococcus formosensis; reclassification

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2021 年 71 卷 8 期

页码：

收录情况： SCI

摘要： Currently, Lactococcus garvieae contains two subspecies: L. garvieae subsp. bovis and L. garvieae subsp. garvieae. In a study by Varsha and Nampoothiri, high pheS (99.7%) and rpoA (99.6%) sequence similarities indicated that L. garvieae subsp. bovis and Lactococcus formosensis probably have a close taxonomic relationship; low pheS (92.2%) and rpoA (97.8%) sequence similarities and relatively low DNA-DNA hybridization value (75.8%) indicated that L. garvieae subsp. bovis and L. garvieae subsp. garvieae probably represent two different species. In the present study, the taxonomic relationships between L garvieae subsp. bovis, L garvieae subsp. garvieae and L formosensis were re-examined based on sequence analyses of 16S rRNA, pheS, recA, rpoA and rpoB genes, average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) values, average amino acid identity (AAI), fatty acid methyl ester analysis and phenotypic characterization. L. garvieae subsp. bovis LMG 30663(T) exhibited 97.3% ANI. 78.3% dDDH and 96.4% AAI values to L. formosensis NBRC 109475(T), higher than the threshold for species demarcation (95-96, 70 and 95-96%, respectively), indicating that L garvieae subsp. bovis LMG 30663(T) and L. formosensis NBRC 109475(T) belong to the same species. L. garvieae subsp. bovis LMG 30663(T) had 91.2% ANI, 43.3% dDDH and 92.9-93.0% AA! values with the type strain of L. garvieae subsp. garvieae, indicating that they represent two different species. Because L. formosensis has been proposed and validated before L garvieae subsp. bovis. L. garvieae subsp. bovis is transferred to L. formosensis as L. formosensis subsp. bovis comb. nov. The type strain of L. formosensis subsp. bovis is BSN307(T) (=DSM 10057(T)=MCC 2824(T) =KCIC 21083(T)=LMG 30663(T)). The type strain of L formosensis subsp. formosensis is 516(T) (=NBRC 109475(T) =BCRC 80576(T)).

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