Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants

文献类型： 外文期刊

第一作者： Zheng, Xiaohong

作者： Zheng, Xiaohong;Peng, Yaxin;Shan, Junhan;Zhang, Ning;Wu, Di;Guo, Jianping;Huang, Jin;Guan, Wei;Shi, Shaojie;Zhou, Cong;Chen, Rongzhi;Du, Bo;Zhu, Lili;Yang, Fang;Yuan, Longping;He, Guangcun;Xin, Yeyun;Fu, Xiqin;Yuan, Longping

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关键词： brown planthopper; rice plants; RNA-seq; lipidomics; lipid metabolism; RNA interference

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

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收录情况： SCI

摘要： The brown planthopper (BPH) (Nilaparvata lugens Stal) is a highly destructive pest that seriously damages rice (Oryza sativa L.) and causes severe yield losses. To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice, we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant (NIL-Bph6 and NIL-Bph9) plants or a wild-type, BPH susceptible (9311) plant. Insects that were fed on resistant rice transformed triglyceride (TG) to phosphatidylcholine (PC) and digalactosyldiacylglycerol (DGDG), with these lipid classes showing significant alterations in fatty acid composition. Moreover, the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes. Knockdown of the NlBmm gene, which encodes a lipase that regulates the mobilization of lipid reserves, significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs. Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes. Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation, body maintenance, growth and oviposition.

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