文献类型： 外文期刊
第一作者： Li, Zhaocai
作者： Li, Zhaocai;Liu, Ping;Zhou, Jizhang
作者机构：
关键词： Chlamydia; Infectivity; Lipofectamine; Centrifugation
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）
ISSN： 0003-2697
年卷期： 2020 年 610 卷
页码：
收录情况： SCI
摘要： Cultivation of Chlamydia species in cell lines requires centrifugation of the inoculum onto diethylaminoethyl-dextran-pretreated cell monolayers to improve the infection efficiency. Here we report that the addition of DNA transfection reagent Lipofectamine in the inoculum significantly enhances the infectivity of Chlamydia abortus in mouse fibroblast McCoy cells, with an infection efficiency equivalent to that of the centrifugation method. Similar enhancement effects of Lipofectamine on the infectivity of C. psittaci and C. trachomatis were also observed. This study provides an alternative and convenient method for the cultivation of Chlamydia species in vitro in the absence of centrifugation.
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