Rapid determination of furosine in milk using microwave-assisted HCl hydrolysis and ultra-performance liquid chromatography

文献类型： 外文期刊

第一作者： Wang, Fengen

作者： Wang, Fengen;Zhang, Yangdong;Wen, Fang;Zheng, Nan;Wang, Jiaqi;Wang, Fengen;Wang, Jiaqi;Wang, Fengen;Wang, Fengen;Zhang, Yangdong;Wen, Fang;Zheng, Nan;Wang, Jiaqi

作者机构：

关键词： furosine; milk; microwave-assisted HCl hydrolysis; ultra-performance liquid chromatography

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 12 期

页码：

收录情况： SCI

摘要： Because the conventional methods for furosine analy-sis are time-consuming, a modified method is presented to improve analysis efficiency. Microwave-assisted HCl hydrolysis was performed at 140, 150, and 160 degrees C for 10 to 200 min, with 6, 8, or 9 M HCl. The hydrolysate purification process was carried out using only paper and membrane filtration. An ultra-performance liquid chromatography (UPLC) system was used to achieve rapid analysis of furosine. The results showed that microwave-assisted HCl hydrolysis at 8 M and 160 degrees C led to a stable furosine yield and took only 40 min. The UPLC analysis was completed in 8 min. The modified method was validated and obtained limit of detection at 3 mu g/L, limit of quantitation of 10 mu g/L, linearity range of 0.2 to 5.0 mg/L, 80.5 to 94.2% recoveries from spiked samples, and coefficients of variation of 2.2 to 6.8%. The modified method is rapid and reliable for the determination of furosine in milk.

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