Integration of gene expression profile data to screen and verify immune-related genes of chicken erythrocytes involved in Marek's disease virus

文献类型： 外文期刊

第一作者： Jahejo, Ali Raza

作者： Jahejo, Ali Raza;Jia, Fa-Jie;Ahsan, Anam;Niu, Sheng;Ning, Guan-Bao;Zhang, Ding;Bi, Yu-Hai;Wang, Qi-Hui;Tian, Wen-Xia;Bukhari, Sayyad Ali Raza;Raza, Sayed Haidar Abbas;Shah, Mujahid Ali;Rajput, Nasir;Bi, Yu-Hai;Wang, Qi-Hui;Han, Ling-Xia

作者机构：

关键词： Chicken; Erythrocytes; Immunity; MDV; TLRs

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2020 年 148 卷

页码：

收录情况： SCI

摘要： Chicken erythrocytes participated in immunity, but the role of erythrocytes in the immunity of Marek's disease virus (MDV) has not been reported related to the immunity genes. The purpose of this study was to screen and verify the immune-related genes of chicken erythrocytes which could be proven as a biomarker in MDV. The datasets (GPL8764-Chicken Gene Expression Microarray) were downloaded from the GEO profile database for control and MDV infected chickens to obtain differentially expressed genes (DEGs) through bioinformatics methods. Kyoto Encyclopedia of Genes and Genomes (KEGG) was performed to find enriched pathways, including Gene Ontology (GO). Based on enriched pathways, the top 19 immune-related genes were screened-out and process further to construct the protein-protein interaction (PPI) networks. The screened genes were validated on RT-PCR and qPCR. Results suggested that the mRNA transcription of Toll-like receptors 2, 3, 4, 6 (TLR2, TLR3, TLR4, TLR6), major histocompatibility complex-II (MHCII), interleukin-7 (IL-7), interferon-beta eta (IFN-beta), chicken myelomonocytic growth factor (cMGF) and myeloid differentiation primary response 88 (MyD88) were significantly up-regulated. The expression of toll-like receptor 5, 7 (TLR5, TLR7) interleukin-12 (IL-12 p40), interleukin-13 (IL-13), and interferon-alpha lpha (IFN-alpha) were significantly down-regulated in the erythrocytes of the infected group (P < 0.05). In contrast, the expression of toll-like receptor-1, 15, 21 (TLR1, TLR15, TLR21), major histocompatibility complex I (MHCI) and Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) were not significant. In conclusion, it has been verified on qRT-PCR results that 19 immune-related genes, which included TLRs, cytokines and MHC have immune functions in MDV infected chickens.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序