Dimethylformamide Inhibits Fungal Growth and Aflatoxin B-1 Biosynthesis in Aspergillus flavus by Down-Regulating Glucose Metabolism and Amino Acid Biosynthesis

文献类型： 外文期刊

第一作者： Pan, Lin

作者： Pan, Lin;Jin, Jing;Xing, Fuguo;Chang, Peng;Yang, Qingli

作者机构：

关键词： aflatoxins; Aspergillus flavus; dimethylformamide; mechanism; glucose metabolism; amino acid biosynthesis

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2020 年 12 卷 11 期

页码：

收录情况： SCI

摘要： Aflatoxins (AFs) are secondary metabolites produced by plant fungal pathogens infecting crops with strong carcinogenic and mutagenic properties. Dimethylformamide (DMF) is an excellent solvent widely used in biology, medicine and other fields. However, the effect and mechanism of DMF as a common organic solvent against fungal growth and AFs production are not clear. Here, we discovered that DMF had obvious inhibitory effect against A. flavus, as well as displayed complete strong capacity to combat AFs production. Hereafter, the inhibition mechanism of DMF act on AFs production was revealed by the transcriptional expression analysis of genes referred to AFs biosynthesis. With 1% DMF treatment, two positive regulatory genes of AFs biosynthetic pathway aflS and aflR were down-regulated, leading to the suppression of the structural genes in AFs cluster like aflW, aflP. These changes may be due to the suppression of VeA and the subsequent up-regulation of FluG. Exposure to DMF caused the damage of cell wall and the dysfunction of mitochondria. In particular, it is worth noting that most amino acid biosynthesis and glucose metabolism pathway were down-regulated by 1% DMF using Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Taken together, these RNA-Seq data strongly suggest that DMF inhibits fungal growth and aflatoxin B-1 (AFB(1)) production by A. flavus via the synergistic interference of glucose metabolism, amino acid biosynthesis and oxidative phosphorylation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序