Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers

文献类型： 外文期刊

第一作者： Wang Ping

作者： Wang Ping;Chen Zhi-qiang;Wang Ping;Bai Yu-lu;Wang Min-xia;Hu Bin-hua;Pu Zhi-gang;Zhang Zhi-yong;Zhang Qiong;Xu Deng-wu;Luo Wen-long

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关键词： breeding efficiency; maintainer of cytoplasmic male sterility; gene pyramiding; molecular marker-assisted selection

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 12 期

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收录情况： SCI

摘要： The integrated use of molecular marker-assisted selection (MAS) and anther culture has potential to significantly increase efficiency in plant breeding; however, reports on this kind of practical use are very limited. In the present study, we report the development of cytoplasmic male sterile (CMS) maintainers with aroma, disease resistance and red-brown hulls, as an example of integration of MAS and anther culture in rice breeding. A high-resolution melting (HRM)-based functional molecular marker was developed for the red-brown hull trait caused by a unique mutation (rbh1) in OsCAD2. Functional molecular markers for genes of rice blast resistance (Pi2), aroma (fgr) and red-brown hull (rbh1) were used for precise genotyping of individual plants in the BC1 and BC2F2 populations derived from a cross between CMS maintainers Huaxiang B (pi2-/rbh1-/fgr-) and Rong 3B (Pi2+/RBH1+/Fgr+). A total of 89 doubled haploid (DH) lines were generated from selected BC2F2 plants (Pi2+/rbh1-/fgr-) by anther culture. Seven DH lines were subsequently selected as the potential new CMS maintainers based on their overall performance and high resistance to blast. Our study demonstrated that integration of MAS and anther culture significantly accelerated the development of CMS maintainers with multiple stacked genes.

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