Development of elite restoring lines by integrating blast resistance and low amylose content using MAS

文献类型: 外文期刊

第一作者: Xiao Wu-ming

作者: Xiao Wu-ming;Peng Xin;Luo Li-xin;Liang Ke-qin;Wang Jia-feng;Huang Ming;Liu Yong-zhu;Guo Tao;Luo Wen-long;Wang Hui;Chen Zhi-qiang;Yang Qi-yun;Zhu Xiao-yuan

作者机构:

关键词: rice;restoring line;blast resistance;grain quality;MAS

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2018 年 17 卷 1 期

页码:

收录情况: SCI

摘要: Blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance (R) genes, Pi46 and Pita, along with the gene Wx(b), which mainly affects rice endosperm amylose content (AC), were introgressed into an elite indica restoring line, R8166, which has little blast resistance and poor grain quality through marker-assisted selection (MAS). Eight improved lines were found to have recurrent genome recovery ratios ranging from 88.68 to 96.23%. Two improved lines, R163 and R167, were selected for subsequent studies. R167, which has the highest recovery ratio (96.23%), showed no significant differences in multiple agronomic traits. In contrast, R163 with the lowest recovery ratio (88.68%) exhibited significant differences in heading date and yield per plant compared with the recurrent parent. At two developmental stages, R163 and R167 had greatly enhanced resistance to blast over the recurrent parent. Similar trends were also observed for agronomic traits and blast resistance in R163-and R167-derived hybrids when compared with the counterparts from R8166. In addition, R163, R167, and their derived hybrids significantly improved the grain quality traits, including amylose content (AC), gel consistency (GC), chalky grain rate (CGR), and degree of endosperm chalkiness (DEC). It confirmed the success of efficiently developing elite restoring lines using MAS in this study.

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