Response of chemokine receptors CXCR2 and integrin beta 2 after Streptococcus agalactiae and Aeromonas hydrophila challenge in GIFT strain of Nile tilapia Oreochromis niloticus

文献类型： 外文期刊

第一作者： Hu, Qiaomu

作者： Hu, Qiaomu;Ao, Qiuwei;Zhu, Jiajie

作者机构：

关键词： Tilapia; Cxcr2; Integrin beta 2; Expression; Immune response

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2021 年 115 卷

页码：

收录情况： SCI

摘要： CXCR2 is a G-protein-coupled cell surface chemokine receptor, and integrins are heterodimeric transmembrane (TM) glycoproteins. These proteins work together to activate neutrophils in the immune defense, but knowledge of their function in tilapia is limited. RACE technology was used to clone the full length of the Nile tilapia Oreochromis niloticus Cxcr2 gene, which included a 954 bp open reading frame encoding 318 amino acids, and the integrin beta 2 gene, with a 2373 bp open reading frame and 791 amino acids. Sequence analyses showed that Cxcr2 and integrin beta 2 are conserved among species. Expression profile was performed using qRT-PCR and indicated that Cxcr2 and integrin beta 2 were distributed throughout the examined organ tissues, with highest expression observed in the immune tissues. Expression of Cxcr2 and integrin beta 2 were increased after challenged with Streptococcus agalactiae or Aeromonas hydrophila. Results suggest that Cxcr2 and integrin beta 2 genes play a role in immune response in Nile tilapia and provide basic data for molecular-assistant selection of disease-resistant bloodstock to improve the production.

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