Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterusalbus

文献类型： 外文期刊

第一作者： Hu, Qiaomu

作者： Hu, Qiaomu;Xia, Xueping;Lian, Zitong;Tian, Haifeng;Li, Zhong

作者机构：

关键词： Monopterusalbus; LncRNA; DNA methylation; Sex reversal; Gene expression

期刊名称：BIOLOGY OF SEX DIFFERENCES （ 影响因子：7.9； 五年影响因子：6.9 ）

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年卷期： 2023 年 14 卷 1 期

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收录情况： SCI

摘要： Background Monopterusalbus is a hermaphroditic fish with sex reversal from ovaries to testes via the ovotestes in the process of gonadal development, but the molecular mechanism of the sex reversal was unknown. Methods We produced transcriptomes containing mRNAs and lncRNAs in the crucial stages of the gonad, including the ovary, ovotestis and testis. The expression of the crucial lncRNAs and their target genes was detected using qRT-PCR and in situ hybridization. The methylation level and activity of the lncRNA promoter were analysed by applying bisulfite sequencing PCR and dual-luciferase reporter assays, respectively. Results This effort revealed that gonadal development was a dynamic expression change. Regulatory networks of lncRNAs and their target genes were constructed through integrated analysis of lncRNA and mRNA data. The expression and DNA methylation of the lncRNAs MSTRG.38036 and MSTRG.12998 and their target genes Psm beta 8 and Ptk2 beta were detected in developing gonads and sex reversal gonads. The results showed that lncRNAs and their target genes exhibited consistent expression profiles and that the DNA methylation levels were negatively regulated lncRNA expression. Furthermore, we found that Ptk2 beta probably regulates cyp19a1 expression via the Ptk2 beta/EGFR/STAT3 pathway to reprogram sex differentiation. Conclusions This study provides novel insight from lncRNA to explore the potential molecular mechanism by which DNA methylation regulates lncRNA expression to facilitate target gene transcription to reprogram sex differentiation in M.albus, which will also enrich the sex differentiation mechanism of teleosts. Highlights center dot Construction of the lncRNA-mRNA interaction network in the sex differentiation.center dot DNA methylation was negatively associated with lncRNA expression in developing gonads.center dot Ptk2 beta regulate Ptk2 beta/EGFR/STAT3 pathway to potentially regulate sex differentiation.

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