The role of PA-X C-terminal 20 residues of classical swine influenza virus in its replication and pathogenicity

文献类型： 外文期刊

第一作者： Wang, Xiu-Hui

作者： Wang, Xiu-Hui;Gong, Xiao-Qian;Ruan, Bao-Yang;Yu, Ling-Xue;Liu, Xiao-Min;Wang, Qi;Wang, Shuai-Yong;Wang, Juan;Zhang, Yi-Feng;Zhou, Yan-Jun;Shan, Tong-Ling;Tong, Wu;Zheng, Hao;Kong, Ning;Yu, Hai;Tong, Guang-Zhi;Wang, Xiu-Hui;Zhang, Yi-Feng;Wen, Feng;Yu, Hai;Yu, Hai;Tong, Guang-Zhi

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关键词： Swine influenza virus; PA-X; C-Terminal 20 residues; Replication and pathogenicity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2020 年 251 卷

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收录情况： SCI

摘要： PA-X is a fusion protein encoded by a +1 frameshifted open reading frame (X-ORF) in PA gene. The X-ORF can be translated in full-length (61 amino acids, aa) or truncated (41 aa) form. However, the role of C-Terminal 20 aa of PA-X in virus function has not yet been fully elucidated. To this end, we constructed the contemporary influenza viruses with full and truncated PA-X by reverse genetics to compare their replication and pathogenicity. The fulllength PA-X virus in MDCK and human A549 cells conferred 10to 100-fold increase in viral replication, and more virulent and caused more severe inflammatory responses in mice relative to corresponding truncated PA-X virus, suggesting that the terminal 20 aa could play a role in enhancing viral replication and contribute to virulence.

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