Molecular characterization and expression pattern of inhibin alpha and beta b in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Zhang, Ning

作者： Zhang, Ning;Dong, Zhongdian;Zhang, Ning;Dong, Zhongdian;Xu, Wenteng;Cui, Zhongkai;Wang, Qian;Chen, Songlin;Zhang, Ning;Dong, Zhongdian;Xu, Wenteng;Cui, Zhongkai;Wang, Qian;Chen, Songlin

作者机构：

关键词： Inhibins; Cynoglossus semilaevis; Expression pattern; Hybridization

期刊名称：GENE EXPRESSION PATTERNS （ 影响因子：1.224； 五年影响因子：1.478 ）

ISSN： 1567-133X

年卷期： 2020 年 38 卷

页码：

收录情况： SCI

摘要： Inhibin plays important roles in vertebrate reproduction and development. In this study, we have cloned two genes encoding inhibin subunits, inh alpha and ihn beta b, in Chinese tongue sole. inh alpha consists of 1032 bp, encoding a 343 amino-acid protein. inh beta b is composed of 1275 bp, encoding a 424 amino-acid protein. Phylogenetic tree analysis indicated that INH alpha and INFI beta B were independently evolved. qPCR showed that inh alpha expression of in male testis was higher than that in ovary and pseudomale testis, while the expression of inh beta b in ovary was higher than that in male and pseudomale testis. During gonadal developmental stages, inh alpha expression reached highest at 120 days post hatching (dph) both in ovary and testis, then showed decline in ovary but it was first decreased and then increased in the testis. Similarly, inh beta b expression in ovary was low at 50-80 dph. At 120 dph, its expression was significantly increased to the peak level, and then gradually decreased. inh beta b expression in testis maintained at a low level. During the embryonic developmental stages, inha displayed the highest expression at 32-cell stage, whereas inh beta b reached the highest expression at blastula stages. In situ hybridization data showed that both of inh alpha and inh beta b were detected in oocytes of all stages. In male testis, inh alpha and inh beta b was localized in spermatogonia, spermatocytes, spermatozoa, sertoli and leydig cells. In pseudomale testis, inh alpha showed the similar pattern in male testis, while the inh beta b was detected in spermatocytes and spermatozoa. These data suggested that inh alpha may participate the spermatogenesis and oogenesis of Chinese tongue sole, while inh beta b might predominantly function in oogenesis.

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