Comparative transcriptome and iTRAQ-based proteome analysis in mature leaves of Brassica carinata provides insights into the purple leaf color diversity
文献类型: 外文期刊
第一作者: Wang, Tianya
作者: Wang, Tianya;Yu, Kunjiang;Khattak, Aimal Nawaz;Tian, Entang;Guo, Shaomin;Jiang, Yingfen;Zou, Jun
作者机构:
关键词: Brassica carinata; anthocyanin; iTRAQ; proteomic analysis; transcriptome analysis
期刊名称:JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY ( 影响因子:1.641; 五年影响因子:1.616 )
ISSN: 1462-0316
年卷期: 2021 年 96 卷 4 期
页码:
收录情况: SCI
摘要: Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of Brassicacarinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed comparative transcriptome and iTRAQ-based proteome technique to investigate the expression profile of the purple-leaved line (BC-P01) in comparison with that of the green-leaved line (BC-G01) in B. carinata. In total, 58,124 transcripts and 4,631 proteins were identified, of which 5,183 and 285 exhibited significant changes in abundance between BC-G01 and BC-P01. Joint transcriptome and proteome analysis reveal six candidate genes of UDP-glucosyl transferase 79B1 (UGT79B1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, UDP-glucosyl transferase 79C1 (UGT75C1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, chalcone isomerase (CHI) and chalcone synthase (CHS) for synthesis of chalcone, anthocyanidin 5-O-glucoside-6-O-malonyltransferase (AT5MAT) for malonylation of anthocyanins, granule bound starch synthase 1 (GBSS1) for amylose starch biosynthetic, glutathione s-transferase f3 (GSTF3) for encoding glutathione transferase for anthocyanin transporting, respectively. The expression level of the transcriptome data was further verified by qRT-PCR method. The study can provide useful information for biosynthesis of total anthocyanin content (TAC) and molecular breeding in B. carinata and other species in Brassiceae.
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