Development of 'multiresistance rice' by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system

文献类型： 外文期刊

第一作者： Li, Chuanxu

作者： Li, Chuanxu;Zhang, Jianguo;Zhou, Fei;Chen, Hao;Lin, Yongjun;Li, Chuanxu;Zhang, Jianguo;Yin, Changxi;Ma, Weihua;Zhou, Fei;Chen, Hao;Lin, Yongjun;Ren, Zhiyong;Yin, Changxi;Ma, Weihua;Xie, Rong

作者机构：

关键词： rice; herbicide; borer; brown planthopper; bacterial blight; rice blast; multigene transformation; pest management

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2021 年 77 卷 3 期

页码：

收录情况： SCI

摘要： Background Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. Results In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate-tolerance gene (I. variabilis-EPSPS*), lepidopteran pest resistance gene (Cry1C*), brown planthopper resistance genes (Bph14* and OsLecRK1*), bacterial blight resistance gene (Xa23*) and rice blast resistance gene (Pi9*) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium-mediated transformation and 'multiresistance rice' (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. Conclusion A multigene transformation strategy was employed to successfully develop rice lines with multiresistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast, and the obtained MRR is expected to have great application potential.

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