Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw

文献类型： 外文期刊

第一作者： Yu, Xiaoxiao

作者： Yu, Xiaoxiao;Li, Jianzhen;Su, Yingjie;Gao, Mingyue;Jin, Tingwei;Chen, Guang;Yu, Xiaoxiao;Li, Jianzhen;Su, Yingjie;Gao, Mingyue;Jin, Tingwei;Chen, Guang;Zhang, Zhaoye

作者机构：

关键词： Co-immobilization enzyme; Cascade reaction; Lignocellulose; Gluconic acid

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2021 年 321 卷

页码：

收录情况： SCI

摘要： The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions.

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