The role of Nrf2 in mitigating cadmium-induced oxidative stress of Marsupenaeus japonicus

文献类型： 外文期刊

第一作者： Ren, Xianyun

作者： Ren, Xianyun;Xu, Yao;Yu, Zhenxing;Liu, Ping;Li, Jian;Ren, Xianyun;Xu, Yao;Yu, Zhenxing;Liu, Ping;Li, Jian;Yu, Zhenxing;Xu, Yao;Mu, Cuimin

作者机构：

关键词： Marsupenaeus japonicas; Nrf2-Keap1 pathway; Oxidative stress; Detoxification; Apoptosis

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：8.071； 五年影响因子：8.35 ）

ISSN： 0269-7491

年卷期： 2021 年 269 卷

页码：

收录情况： SCI

摘要： Nuclear factor-erythroid 2-related factor-2 (Nrf2) is an important modulator of cellular responses against Cd in mammalian cells. However, whether such modulation is conserved in Marsupenaeus japonicas remains unknown. In our study, the shrimps were injected with dsRNA targeting Nrf2 at 4 mu g g(-1) body weight (b.w.) or sulforaphane (SFN) at 5 mu g g(-1) b.w., and then were exposed to 40 mg L-1 CdCl2 for 48 h. After Nrf2 knockdown, the Cd content increased, but decreased in the SFN group. This suggested that Nrf2 could promote Cd excretion. A terminal deoxynulceotidyl transferase nick-end-labeling (TUNEL) assay revealed that the Nrf2 knockdown increased the number of apoptotic cells in M. japonicas, while SFN decreased the number of apoptotic cells. After Nrf2 knockdown, the total antioxidant capacity (T-AOC), superoxide dismutase (Sod) activity, and related gene expression decreased significantly, while the malondialdehyde (MDA) content increased remarkably. By contrast, SFN injection alleviated the oxidative stress, as evidenced by increased T-AOC, Sod activity, sod mRNA expression and a reduced MDA content. Similarly, detoxification related enzyme activities (ethoxyresorufin O-deethylase and glutathione-S-transferase (GST)) and their corresponding gene expressions (cyp3a (cytochrome P450 family 3 subfamily A) and gst) were suppressed in the ds-Nrf2 injection group, while they were elevated in the SFN group. In addition, ds-Nrf2 activated mitochondrial apoptotic pathway, as evidenced the mRNA and protein levels of caspase-3, Bcl2 associated X protein (Bax), and p53, while SFN treatment suppressed them. These results displayed that in M. japonicus Cd-induced cellular oxidative damage probably acts via the Nrf2 pathway. (C) 2020 Elsevier Ltd. All rights reserved.

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