ValSten: a new wild species derived allotetraploid for increasing genetic diversity of the peanut crop (Arachis hypogaea L.)

文献类型： 外文期刊

第一作者： Gao, Dongying

作者： Gao, Dongying;Chavarro, M. Carolina;Xia, Han;Jackson, Scott A.;Bertioli, David J.;Leal-Bertioli, Soraya C. M.;Gao, Dongying;Chavarro, M. Carolina;Xia, Han;Jackson, Scott A.;Bertioli, David J.;Leal-Bertioli, Soraya C. M.;Gao, Dongying;Chavarro, M. Carolina;Bertioli, David J.;Araujo, Ana C. G.;Nascimento, Eliza F. M. B.;Nascimento, Eliza F. M. B.;Xia, Han;Leal-Bertioli, Soraya C. M.

作者机构：

关键词： Wild peanut; Breeding; Polyploidy; Disease resistance; Genomic variation; Arachis

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 2021 年 68 卷 4 期

页码：

收录情况： SCI

摘要： Introgression of desirable traits from wild relatives plays an important role in crop improvement, as wild species have important characters such as high resistance to pests and pathogens. However, use of wild peanut relatives is challenging because almost all wild species are diploid and sexually incompatible with cultivated peanut, which is tetraploid (AABB genome type; 2n = 4x = 40). To overcome the ploidy barrier, we used 2 wild species to make a tetraploid with the same allotetraploid genome composition as cultivated peanut. Crosses were made between 2 diploid wild species, Arachis valida Krapov. and W.C. Greg. (BB genome; 2n = 2x = 20) and Arachis stenosperma Krapov. and W.C. Greg. (AA genome; 2n = 2x = 20). Cuttings from the diploid F-1 AB hybrid were treated with colchicine to induce chromosome doubling thus generating an induced allotetraploid. Chromosome counts confirmed polyploidy (AABB genome; 2n = 4x = 40). We named the new allotetraploid ValSten. Plants had well-developed fertile pollen, produced abundant seed and were sexually compatible with cultivated peanut. ValSten exhibits the same high resistance to early and late leaf spot and rust as its diploid parents. Notably, we observed morphological variations, including flower width and branch angles in the earliest generation (S-0) of allotetraploids. A SNP array was used to genotype 47 S-0 allotetraploids. The great majority of markers showed the additive allelic state from both parents (AABB). However, some loci were AAAA or BBBB, indicating homeologous recombination. ValSten provides a new, vigorous, highly fertile, disease resistant germplasm for peanut research and improvement.

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[1]ValSten: a new wild species derived allotetraploid for increasing genetic diversity of the peanut crop (Arachis hypogaea L.). Gao, Dongying,Chavarro, M. Carolina,Xia, Han,Jackson, Scott A.,Bertioli, David J.,Leal-Bertioli, Soraya C. M.,Gao, Dongying,Chavarro, M. Carolina,Xia, Han,Jackson, Scott A.,Bertioli, David J.,Leal-Bertioli, Soraya C. M.,Gao, Dongying,Chavarro, M. Carolina,Bertioli, David J.,Araujo, Ana C. G.,Nascimento, Eliza F. M. B.,Nascimento, Eliza F. M. B.,Xia, Han,Leal-Bertioli, Soraya C. M.. 2021

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