High-density bin-based genetic map reveals a 530-kb chromosome segment derived from wild peanut contributing to late leaf spot resistance

文献类型： 外文期刊

第一作者： Pan, Jiaowen

作者： Pan, Jiaowen;Li, Xiaojie;Yu, Conghui;Wang, Guanghao;Tian, Ruizheng;Li, Changsheng;Xia, Han;Zhao, Shuzhen;Hou, Lei;Zi, Hailing;Wang, Xingjun;Zhao, Chuanzhi;Pan, Jiaowen;Li, Xiaojie;Yu, Conghui;Tian, Ruizheng;Xia, Han;Zhao, Shuzhen;Hou, Lei;Wang, Xingjun;Zhao, Chuanzhi;Fu, Chun;Song, Xiaofeng;Wang, Zhenyu;Gao, Meng;Bian, Jianxin;Liu, Xiaoqin;Bertioli, David;Leal-Bertioli, Soraya;Pandey, Manish K.

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 3 期

页码：

收录情况： SCI

摘要： Key messageTwenty-eight QTLs for LLS disease resistance were identified using an amphidiploid constructed mapping population, a favorable 530-kb chromosome segment derived from wild species contributes to the LLS resistance.AbstractLate leaf spot (LLS) is one of the major foliar diseases of peanut, causing serious yield loss and affecting the quality of kernel and forage. Some wild Arachis species possess higher resistance to LLS as compared with cultivated peanut; however, ploidy level differences restrict utilization of wild species. In this study, a synthetic amphidiploid (Ipadur) of wild peanuts with high LLS resistance was used to cross with Tifrunner to construct TI population. In total, 200 recombinant inbred lines were collected for whole-genome resequencing. A high-density bin-based genetic linkage map was constructed, which includes 4,809 bin markers with an average inter-bin distance of 0.43 cM. The recombination across cultivated and wild species was unevenly distributed, providing a novel recombination landscape for cultivated-wild Arachis species. Using phenotyping data collected across three environments, 28 QTLs for LLS disease resistance were identified, explaining 4.35-20.42% of phenotypic variation. The major QTL located on chromosome 14, qLLS14.1, could be consistently detected in 2021 Jiyang and 2022 Henan with 20.42% and 12.12% PVE, respectively. A favorable 530-kb chromosome segment derived from Ipadur was identified in the region of qLLS14.1, in which 23 disease resistance proteins were located and six of them showed significant sequence variations between Tifrunner and Ipadur. Allelic variation analysis indicating the 530-kb segment of wild species might contribute to the disease resistance of LLS. These associate genomic regions and candidate resistance genes are of great significance for peanut breeding programs for bringing durable resistance through pyramiding such multiple LLS resistance loci into peanut cultivars.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序