A cotton WAKL protein interacted with a DnaJ protein and was involved in defense against Verticillium dahliae

文献类型： 外文期刊

第一作者： Feng, Hongjie

作者： Feng, Hongjie;Li, Cheng;Zhou, Jinglong;Yuan, Yuan;Feng, Zili;Shi, Yongqiang;Zhao, Lihong;Zhang, Yalin;Wei, Feng;Zhu, Heqin

作者机构：

关键词： Cotton; Verticillium dahliae; Resistance; Phosphorylation; GhWAKL

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 167 卷

页码：

收录情况： SCI

摘要： Accumulating evidence indicates that plant cell wall-associated receptor-like kinases (WAKs) involve in defense against pathogen attack, but their related signaling processes and regulatory mechanism remain largely unknown. We identified a WAK-like kinase (GhWAKL) from upland cotton (Gossypium hirsutum) and characterized its functional mechanism. Expression of GhWAKL in cotton plants was induced by Verticillium dahliae infection and responded to the application of salicylic acid (SA). Knockdown of GhWAKL expression results in the reduction of SA content and suppresses the SA-mediated defense response, enhancing cotton plants susceptibility to V. dahliae. And, ecotopic overexpression of GhWAKL in Arabidopsis thaliana conferred plant resistance to the pathogen. Further analysis demonstrated that GhWAKL interacted with a cotton DnaJ protein (GhDNAJ1) on the cell membrane. Silencing GhDNAJ1 also enhanced cotton susceptibility to V. dahliae. Moreover, the mutation of GhWAKL at site Ser628 with the phosphorylation decreased the interaction with GhDNAJ1 and compromised the plant resistance to V. dahliae. We propose that GhWAKL is a potential molecular target for improving resistance to Verticillium wilt in cotton. (C) 2020 Elsevier B.V. All rights reserved.

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