文献类型： 外文期刊
第一作者： Wang, Yahui
作者： Wang, Yahui;Ren, Ling;Xing, Yishen;Hu, Xin;Li, Qian;Xu, Lingyang;Li, Junya;Zhang, Lupei;Wang, Yahui;Ren, Ling;Xing, Yishen;Hu, Xin;Li, Qian;Xu, Lingyang;Li, Junya;Zhang, Lupei;Hu, Xin
作者机构：
关键词： BMP4; Rosiglitazone; Fetal bovine muscle; Progenitor cells; Intramuscular adipogenesis
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2021 年 53 卷 1 期
页码：
收录情况： SCI
摘要： Intramuscular fat (IMF) content is one of the most important factors determining beef quality and price. Intramuscular adipocytes develop from mesenchymal stem cells (MSCs) in mesoderm. The mechanisms of preadipocytes differentiate into mature adipocytes to a great extent are clear, but the commitment of MSCs to preadipocytes is largely unknown. In this study, the Platelet-derived growth factor receptor alpha (PDGFR alpha) positive progenitor cells were isolated from the longissimus dorsi muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated cells. The result indicated that BMP4 and rosiglitazone could promote adipogenesis and be applied in adipogenic differentiation of fetal bovine derived progenitor cells.
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