Comparative Analysis of Muscle Development in Zebrafish with Different Intermuscular-Bones Patterns

文献类型： 外文期刊

第一作者： Yang, Jian

作者： Yang, Jian;Tong, Guangxiang;Sun, Zhipeng;Zheng, Xianhu;Lv, Weihua;Cao, Dingchen;Sun, Xiaowen;Kuang, Youyi

作者机构：

关键词： Zebra fish; Intermuscular-bones; Muscle development; Muscle-specific genes; Muscle fiber

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2021 年 53 卷 1 期

页码：

收录情况： SCI

摘要： In this study, we used an intermuscular-bones (imbs) partial deletion zebrafish (Danio rerio) mutant to analyze the impact of imbs deficiency on adjacent muscle development and growth by examining expression of muscle-specific genes and muscle structure. Five muscle-specific genes including myod, myog, mef5, mef2ca and sox6 were selected to test and verify the expression differences in embryonic development stages (3 hpf, 6 hpf, 12 hpf, 24 hpf, and 72 hpf) and post-embryonic stages (15 dpf, 30 dpf, 45 dpf, 60 dpf and 75 dpf). Compared to the wild-type (WT) siblings, the mutants showed no significant differences in the 5 gene expressions. Among different development stages, the expression levels and patterns of the 5 genes in the mutants were similar to that of WT zebrafish in both embryonic and post-embryonic development stages. Furthermore, the results of histological analyses of the muscle fiber showed that there were no significant differences in muscle fiber density between imbs mutant and WT zebrafish, and no significant differences between anterior and posterior part to the dorsal fin at the same developmental stages. In WT and mutants, the density of muscle fiber declined gradually over time. In conclusion, the lack of intermuscular-bones has no influence on adjacent muscle development.

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