Quantitative determination of mutton adulteration with single-copy nuclear genes by real-time PCR

文献类型： 外文期刊

第一作者： Li, Tingting

作者： Li, Tingting;Wang, Jishi;Wang, Zhiying;Qiao, Lu;Liu, Rui;Chen, Ailiang;Li, Shanshan

作者机构：

关键词： Real-time PCR; Single-copy genes; Adulterative; Mutton

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 344 卷

页码：

收录情况： SCI

摘要： Mitochondrial genes were generally adopted for PCR-based meat adulteration authentication due to their excellent specificity to species and numerous copies in one cell. However, the number of mitochondrial gene copies varies according to cells and tissues, which leads to quantification errors for meat adulteration. To address this problem, single-copy nuclear genes were selected to develop a quantitative method for identifying mutton adulteration in this study. Both single-copy genes specific to sheep species and single-copy reference genes show good linearity between Ct values and series diluted DNA concentrations, with the correlation coefficients of 0.9999 and 0.9993, respectively. Meanwhile, a constant (correction factor) was introduced to transform DNA concentrations into mutton proportions in adulterated meat. With this method, simulated mutton-pork, mutton-chicken and mutton-duck adulteration samples could be accurately quantified with the recovery rates of 89.56%, 107.13% and 95.20%, respectively.

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