Integrative analyses of transcriptome and metabolome shed light on the regulation of secondary metabolites in pseudobulbs of two Bletilla striata (Thunb.) Reichb.f. varieties

文献类型： 外文期刊

第一作者： Chen, Jin

作者： Chen, Jin;Zhu, Feiying;Liu, Ling;Xia, Shitou;Chen, Jin;Yi, Lixiong;Dai, Yanjiao;Chen, Shan;He, Aiguo

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关键词： Blettila striata; Fsendobulb; Transcriptomics; Metabolomics

期刊名称：JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS （ 影响因子：3.4； 五年影响因子：3.631 ）

ISSN：

年卷期： 2021 年 20 卷

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收录情况： SCI

摘要： Blettila striata (Thunb.) Reichb .f. is an important ornamental and herbaceous medicinal plant. However, natural resources are endangered and breeding and improvement of the medicinal plant using modern biotechnology are urgently needed. Increasing the harvest biomass is critical to the total yield of bioactive substances. The 'MBS' variety of Bletilla striata could be an ideal parental line for genetic and metabolic engineering as the yield of the 'MBS' was more than that of the 'BS'. Nonetheless, its genetic background and metabolic networks remain largely unknown. In this study, integrative analyses of transcriptome and metabolome in pseudobulbs were conducted. Our study establishes a correlation between transcriptome and metabolome in pseudobulbs of Bletilla striata, shedding light on the synthesis pathways in bioactive substances. In total, 11,277 unigenes were differentially expressed in the pseudobulbs of 'MBS' and 'BS'. Moreover, 109 metabolites were identified as differentially accumulated substances. Correlation analysis between differentially expressed genes and differentially accu- mulated metabolites revealed that genes involved in metabolism might contribute to the different levels of polysaccharides and flavonoids between the two varieties. This study should be helpful in bioresource utilization and crop improvement via genetic and metabolic engineering.

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