alpha-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy

文献类型： 外文期刊

第一作者： Chen, Zhilong

作者： Chen, Zhilong;Li, Chen;Yuan, Anwen;Gu, Ting;Zhang, Feng;Wu, Xiaosong;Yang, Qing;Fan, Xiujun;Xiong, Xingyao

作者机构：

关键词： α -solanine; trophoblast cell; toxicity; apoptosis; autophagy

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

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年卷期： 2021 年 13 卷 1 期

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收录情况： SCI

摘要： The trophoblast, an embryonic tissue, exerts a crucial role in the processes of implantation and placentation. Toxins in food can cause malfunction of trophoblasts, resulting in apoptosis, oxidative stress, and abnormal angiogenesis. alpha-solanine, a steroidal glycoalkaloid, has antitumor properties on several cancer cells. However, its effect on human trophoblasts has not been elucidated. In this study, human extravillous trophoblast HTR-8/SVneo cells were exposed to alpha-solanine. Cellular functions including proliferation, migration, invasion, tube formation, and apoptosis were assessed. To monitor autophagic flux, trophoblasts were transfected with a mCherry-GFP-LC3B vector using lentiviral transduction, and expression of autophagy-related biomarkers including Beclin 1, Atgl3, and microtubule-associated protein 1 light chain-3 (MAP1-LC3) were detected. The results show that application of 20 mu M alpha-solanine or above inhibited the cell viability, migration, invasion, and tube formation of the human trophoblast. Cell cycle was arrested at S and G2/M phases in response to 30 mu M alpha-solanine. alpha-solanine induced apoptosis of HTR-8/SVneo cells and triggered autophagy by increasing the autophagic gene expression and stimulating the formation of autophagosome and autophagic flux. In conclusion, alpha-solanine can impair the functions of human trophoblast cells via activation of cell apoptosis and autophagy.

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