Cordyceps cicadae polysaccharides inhibit human cervical cancer hela cells proliferation via apoptosis and cell cycle arrest

文献类型： 外文期刊

第一作者： Xu, Jiao

作者： Xu, Jiao;Tan, Zhi-Cheng;Shen, Zhong-Yuan;Shen, Xing-Jia;Tang, Shun-Ming;Xu, Jiao;Tan, Zhi-Cheng;Shen, Zhong-Yuan;Shen, Xing-Jia;Tang, Shun-Ming

作者机构：

关键词： Artificially cultured Cordyceps cicadae; Polysaccharides; Cell cycle; Apoptosis

期刊名称：FOOD AND CHEMICAL TOXICOLOGY （ 影响因子：6.023； 五年影响因子：5.844 ）

ISSN： 0278-6915

年卷期： 2021 年 148 卷

页码：

收录情况： SCI

摘要： The present study presented the extraction and purification of polysaccharides from artificially cultured Cordyceps cicadae and wild Cordyceps cicadae by pre-soaking ultrasonic water extraction. The effects of different concentrations of polysaccharides on proliferation and cytotoxicity of Hela cells were detected by MTT and LDH methods. The results showed that the proliferation of Hela cells was inhibited by polysaccharides treatment (25 mu g/mL-1600 mu g/mL). The results of flow cytometry further confirmed that polysaccharides blocked the cell cycle in the S phase and promoted apoptosis. RT-qPCR and Western Blot were used to study the mRNA and protein expression of genes related to cell cycle and apoptosis signaling pathway. The results showed that polysaccharides treatment inhibited the expression of Cyclin E, Cyclin A and CDK2 and up regulated the expression of P53. Further, activation of Caspase cascade reaction, up regulation of death receptor, and the ratio of proapoptotic factor/anti-apoptotic factors, thus caused the cell cycle arrest and induced the apoptosis. The above research results lay a foundation for extending the anti-cancer effects of natural plant resources with low toxicity and high efficiency.

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