Comparative Proteomic Analysis of Cucumber Fruits Under Nitrogen Deficiency At the Fruiting Stage

文献类型： 外文期刊

第一作者： Hao, Jia

作者： Hao, Jia;Li, Qiang;Yu, Hongjun;Wang, Heng;Chai, Lin;Miao, Tiantian;Jiang, Weijie

作者机构：

关键词： cucumber; fruit; nitrogen deficiency; proteomics

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）

ISSN： 2095-9885

年卷期： 2021 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Nitrogen deficiency is a major factor that affects the yield and quality of horticultural crops. Differentially expressed proteins were identified in cucumber fruit under nitrogen deficiency treatment, and are expected to be highly useful for dissecting carbon, nitrogen and ascorbate metabolism in cucumber and for improving nitrogen fertilizer utilization. Cucumber plants grown hydroponically in Hoagland solution were transferred to nitrogen-free conditions for 3 or 5 d after the blossom of female flowers. Fruit proteome profiles were assessed using twodimensional electrophoresis. The relative expression of the genes encoding 50 selected differentially expressed proteins and 4 key enzymes in carbon metabolism was analyzed by qRT-PCR. Resulted showed that most of the identified differentially expressed proteins were mainly associated with carbon metabolism, amino acid biosynthesis, ascorbate metabolism, and proteasomes. Glucose phosphorylation was enhanced, while the pentose phosphate pathway was inhibited. Carbon metabolism and the synthesis of most amino acids was affected significantly, and ascorbate might be accumulated under nitrogen deficiency in cucumber fruit.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序