Supplementation with L-kynurenine during in vitro maturation improves bovine oocytes developmental competence through its antioxidative action

文献类型： 外文期刊

第一作者： Lu, Canqiang

作者： Lu, Canqiang;Tang, Zhenhua;Jia, Ruru;Xia, Fan;Yu, Tingzi;Wang, Heng;Mo, Hongfang;Shi, Deshun;Lu, Fenghua;Feng, Yun;Tang, Zhenhua

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关键词： Bovine; Oocytes; In vitro maturation; L-kynurenine; Antioxidant

期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）

ISSN： 0093-691X

年卷期： 2025 年 233 卷

页码：

收录情况： SCI

摘要： Oxidative stress impairs the developmental potential of oocytes during in vitro maturation (IVM). L-kynurenine (L-KYN), an endogenous metabolite, exhibits antioxidant, anti-inflammatory and neuroprotective effects. This work aimed to evaluate the potential effects of L-KYN on bovine oocyte IVM and its mechanisms. Different concentrations of L-KYN (0, 10, 50, 100, and 200 mu mol/L) were supplemented to bovine oocyte IVM medium. Results shown a notable enhancement in the oocyte maturation rate and the subsequent zygote cleavage and blastocyst formation rate when the L-KYN concentration reached 100 mu mol/L. Further analysis revealed that this treatment effectively down-regulated expression levels of genes to cumulus cell apoptosis (Bax and Caspase3), up-regulated expression levels of genes to cumulus cell expansion (HAS2, PTX3, and PTGS2) and oocyte antioxidant (GPX4 and CAT). L-KYN supplementation also effectively increased glutathione (GSH) levels, promoted oocytes mitochondrial activity, enhanced DNA repairability, deceased reactive oxygen species (ROS) levels, and reduced apoptosis. In conclusion, adding 100 mu mol/L of L-KYN to IVM medium could enhance bovine oocyte quality and the subsequent embryonic development. L-KYN could be a potential antioxidant supplement for bovine oocyte maturation in vitro.

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