Supplementation with lipoamide during in vitro maturation improves bovine oocyte maturation and subsequent embryonic development: potential link to PI3K/AKT signaling

文献类型： 外文期刊

第一作者： Lu, Canqiang

作者： Lu, Canqiang;Ruan, Ziyun;Wang, Yun;Tang, Zhenhua;Zhou, Dongping;Yang, Yiqing;Chen, Yanyu;Ren, Jie;Zeng, Chengxi;Shi, Deshun;Lu, Fenghua;Ruan, Ziyun;Tang, Zhenhua;Li, Zhengda

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关键词： Bovine; Oocyte; Somatic cell nuclear transfer; Lipoamide; PI3K/AKT signaling pathway; Antioxidant

期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）

ISSN： 0093-691X

年卷期： 2025 年 243 卷

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收录情况： SCI

摘要： Oxidative stress during oocyte in vitro maturation (IVM) is still concerned. Lipoamide (LAM) has been widely studied as an agent for alleviating various diseases associated with oxidative disruption. This work aimed to evaluate the potential effects of LAM on bovine oocyte IVM and its mechanisms. Different concentrations of LAM (0, 10, 50, 100, and 200 mu mol/L) were supplemented to bovine oocyte IVM medium. The IVF derived zygote cleavage and blastocyst formation rate in the 100 mu mol/L LAM treatment group was increased compared with the control group (P < 0.05).There was no statistical difference in PBI between 100 mu mol/L LAM treatment and the control group, although the treatment tended to increase it (P = 0.059). Further revealed that LAM increased the expression of PI3K and phosphorylated-AKT1 (pAKT1), improved mitochondrial profile, and reduced apoptosis in bovine oocytes. Meanwhile, the reactive oxygen species (ROS) as well as the 8-Hydroxydeoxyguanosine (8-OHdG, DNA damage-specific marker) displayed lower levels accumulation in LAM-exposed oocytes. Taken together, the results show that administration of LAM (100 mu mol/L) during IVM can ameliorate the developmental competence of bovine oocyte through the potential regulation of oxidative stress, apoptosis, DNA damage, and PI3K/AKT signaling.

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