Response of transgenic Arabidopsis expressing maize C-4 photosynthetic enzyme genes to high light

文献类型： 外文期刊

第一作者： Zhang, Qingchen

作者： Zhang, Qingchen;Qi, Xueli;Xu, Weigang;Li, Yan;Zhang, Yu;Peng, Chaojun;Fang, Yuhui;Zhang, Qingchen

作者机构：

关键词： High light; Arabidopsis; chlorophyll a fluorescence; pepc; nadp-ME; ppdk

期刊名称：PLANT SIGNALING & BEHAVIOR （ 影响因子：2.247； 五年影响因子：2.369 ）

ISSN： 1559-2316

年卷期： 2021 年 16 卷 4 期

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收录情况： SCI

摘要： This study assessed the responses of wild-type (WT) and transgenic Arabidopsis expressing seven combinations of maize (Zea mays) genes phosphoenolpyruvate carboxylase (pepc), pyruvate phosphate dikinase (ppdk), and NADP-malic enzyme (nadp-me) to high light. Our results showed that the net CO2 assimilation rate (P-n) and shoot dry weight of four of the transgenic Arabidopsis genotypes were significantly different from those of WT under high-light treatment, being in the order of Zmpepc+Zmppdk+Zmnadp-me (PC-K-M) > Zmpepc+Zmppdk (PC-K) > Zmpepc (PC), Zmpepc+Zmnadp-me (PC-M) > WT. The other genotypes did not differ from WT. This indicated that Zmpepc was essential for maintaining high photosynthetic performance under high light, Zmppdk had a positive synergistic effect on Zmpepc, and the combination of all three genes had the greatest synergistic effect. These four genotypes also maintained higher photosystem II (PSII) activity (K-phase, J-phase, RC/CSm), electron transfer capacity (J-phase), and photochemical efficiency (TRo/ABS), and accumulated less reactive oxygen species (O-2 center dot(-), H2O2) and suffered less damage to the membrane system (MDA) than WT under high light. Collectively, PC, PC-K, PC-M, and PC-K-M used most of the absorbed energy for CO2 assimilation through a significantly higher P-n, which reduced the generation of excess electrons in the photosynthetic apparatus, thereby reducing damage to the membrane system and PSII. This ultimately resulted in improved high-light tolerance. P-n was the main reason for the significant difference in the high-light tolerance of the four genotypes. Joint expression of the three maize genes may be of great value in the genetic improvement of high-light tolerance in C-3 crops.

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