High expression of BCL6 inhibits the differentiation and development of hematopoietic stem cells and affects the growth and development of chickens

文献类型： 外文期刊

第一作者： Li, Hongmei

作者： Li, Hongmei;Hu, Bowen;Hu, Shang;Luo, Wen;Yang, Minmin;Liao, Zhiying;Wei, Haohui;Zhao, Changbin;Li, Dajian;Luo, Qingbin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Li, Hongmei;Hu, Bowen;Hu, Shang;Luo, Wen;Yang, Minmin;Liao, Zhiying;Wei, Haohui;Zhao, Changbin;Li, Dajian;Luo, Qingbin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Li, Hongmei;Hu, Bowen;Hu, Shang;Luo, Wen;Yang, Minmin;Liao, Zhiying;Wei, Haohui;Zhao, Changbin;Li, Dajian;Luo, Qingbin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Li, Hongmei;Hu, Bowen;Hu, Shang;Luo, Wen;Yang, Minmin;Liao, Zhiying;Wei, Haohui;Zhao, Changbin;Li, Dajian;Luo, Qingbin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Sun, Donglei;Shi, Meiqing

作者机构：

关键词： BCL6; Hematopoietic stem cells; Mitochondrial function; Runting and stunting syndrome; Sex-linked dwarf chickens

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：5.032； 五年影响因子：5.922 ）

ISSN： 1674-9782

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundB-cell CLL/lymphoma 6 (BCL6) is a transcriptional master regulator that represses more than 1200 potential target genes. Our previous study showed that a decline in blood production in runting and stunting syndrome (RSS) affected sex-linked dwarf (SLD) chickens compared to SLD chickens. However, the association between BCL6 gene and hematopoietic function remains unknown in chickens.MethodsIn this study, we used RSS affected SLD (RSS-SLD) chickens, SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells (HSCs), to investigate the effect of the BCL6 on differentiation and development of HSCs.ResultsThe results showed that comparison of RSS-SLD chickens with SLD chickens, the BCL6 was highly expressed in RSS-SLD chickens bone marrow. The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow, bone density was reduced, and the levels of immature erythrocytes in peripheral blood were increased. At the same time, the hematopoietic function of HSCs decreased in RSS-SLD chickens, which was manifested by a decrease in the hematopoietic growth factors (HGFs) EPO, SCF, TPO, and IL-3, as well as hemoglobin alpha (1) and hemoglobin beta expression. Moreover, mitochondrial function in the HSCs of RSS-SLD chickens was damaged, including an increase in ROS production, decrease in ATP concentration, and decrease in mitochondrial membrane potential (Delta Psi m). The same results were also observed in SLD chickens compared with normal chickens; however, the symptoms were more serious in RSS-SLD chickens. Additionally, after overexpression of the BCL6 in primary HSCs, the secretion of HGFs (EPO, SCF, TPO and IL-3) was inhibited and the expression of hemoglobin alpha (1) and hemoglobin beta was decreased. However, cell proliferation was accelerated, apoptosis was inhibited, and the HSCs entered a cancerous state. The function of mitochondria was also abnormal, ROS production was decreased, and ATP concentration and Delta Psi m were increased, which was related to the inhibition of apoptosis of stem cells.ConclusionsTaken together, we conclude that the high expression of BCL6 inhibits the differentiation and development of HSCs by affecting mitochondrial function, resulting in impaired growth and development of chickens. Moreover, the abnormal expression of BCL6 might be a cause of the clinical manifestations of chicken comb, pale skin, stunted growth and development, and the tendency to appear RSS in SLD chickens.

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