A Single-Nucleotide Mutation in a GLUTAMATE RECEPTOR-LIKE Gene Confers Resistance to Fusarium Wilt in Gossypium hirsutum

文献类型： 外文期刊

第一作者： Liu, Shiming

作者： Liu, Shiming;Zhang, Xiaojun;Xiao, Shenghua;Qin, Tao;Xu, Zheng;Wang, Tianyi;Wang, Yujing;Zhang, Zhennan;Li, Jianying;Zhang, Xianlong;Zhu, Longfu;Ma, Jun;Shi, Weijun;Kong, Jie;Aierxi, Alifu;Xi, Hui;Nie, Xinhui;You, Chunyuan;Yu, Yu;Lindsey, Keith;Klosterman, Steven J.

作者机构： Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China;Xinjiang Acad Agr Sci, Econ Crop Res Inst, Urumqi 830091, Xinjiang, Peoples R China;Shihezi Univ, Coll Agr, Key Lab Oasis Ecol Agr Xinjiang Bingtuan, Shihezi 832000, Xinjiang, Peoples R China;Shihezi Acad Agr Sci, Cotton Res Inst, Shihezi 832000, Xinjiang, Peoples R China;Xinjiang Acad Agr & Reclamat Sci, Cotton Res Inst, Shihezi 832000, Xinjiang, Peoples R China;Univ Durham, Dept Biosci, Durham DH1 3LE, England;USDA ARS, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA

关键词： disease‐ resistant genes; Fusarium wilt; GLUTAMATE RECEPTOR‐ LIKE genes; Gossypium hirsutum

期刊名称：ADVANCED SCIENCE （ 2020影响因子：16.806； 五年影响因子：17.835 ）

ISSN：

年卷期： 2021 年 8 卷 7 期

页码：

收录情况： SCI

摘要： Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW-resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease-resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR-LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR-based DNA marker (GhGLR4.8(SNP(A/C))) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9-mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8(A) results in a hypersensitive response. This first report of a GLR-encoding gene that functions as an R gene provides a new insight into plant-pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7.

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