Dietary RNAi toxicity assay suggests alpha and gamma subunits of HvCOPI as novel molecular targets for Henosepilachna vigintioctopunctata, an emerging coccinellid pest

文献类型： 外文期刊

第一作者： Lu, Jing

作者： Lu, Jing;Liu, Zhuoqi;Guo, Mujuan;Chen, Shimin;Qiu, Baoli;Pan, Huipeng;Yang, Chunxiao;Velez, Ana M.;Zhang, Youjun;Zhou, Xuguo

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关键词： Henosepilachna vigintioctopunctata; HvCOPIs; Dietary RNAi; Bacterially expressed dsRNA; Feeding cessation

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：5.918； 五年影响因子：5.955 ）

ISSN： 1612-4758

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收录情况： SCI

摘要： Henosepilachna vigintioctopunctata is a highly polyphagous pest of solanaceous plants in Asia and has been predominantly controlled using synthetic insecticides. The intensive use of insecticides has led to the development of resistance, creating an urgent need for new control strategies, for this insect pest. RNA interference (RNAi) has been explored as a pest management strategy in multiple insect pests. In this study, we explored RNAi as a potential alternative for controlling H. vigintioctopunctata. Based on preliminary research, we hypothesized that HvCOPI, a coat protein complex that facilitates retrograde transport, is a promising novel molecular target for H. vigintioctopunctata control. To test this overarching hypothesis, we examined the toxicity of double-stranded RNAs (dsRNAs) towards H. vigintioctopunctata using dietary RNAi toxicity assays targeting eight HvCOPI subunits, including alpha-, beta-, beta '-, gamma-, delta-, epsilon-, zeta-, and Arf1-COPI using in vitro synthesized and bacterially expressed dsRNAs. The results demonstrated that ingestion of dsHvCOPIs induced acute feeding cessation in both larvae and adults, which led to significant mortality. In this study, H. vigintioctopunctata showed similar sensitivity to both types of dsHvCOPIs production. Ingestion of bacterially expressed dsHv alpha COPI and dsHv gamma COPI led to the highest mortality in both larvae and adults. Interestingly, there was a significant positive relationship between the knockdown efficiency of different HvCOPI subunits and the corresponding mortality on day 2 and 4, respectively. These combined results suggest that alpha and gamma subunits of HvCOPI are promising targets for the development of RNAi-based biopesticides for the management of H. vigintioctopunctata.

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