Two carboxylesterase genes in Plutella xylostella associated with sex pheromones and plant volatiles degradation

文献类型： 外文期刊

第一作者： Wang, Mei-Mei

作者： Wang, Mei-Mei;Long, Gui-Jun;Guo, Huan;Liu, Xuan-Zheng;Wang, Hong;Liu, Kun;Zhang, Qiu-Liang;Ma, Yun-Feng;He, Peng;He, Ming;Dewer, Youssef;Li, Zhao-Qun

作者机构：

关键词： carboxylesterase; odorant degrading enzyme; sex pheromone; Plutella xylostella; kinetic parameter

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2021 年 77 卷 6 期

页码：

收录情况： SCI

摘要： BACKGROUND Carboxyl/cholinesterases (CCEs) are thought to play a pivotal role in the degradation of sex pheromones and plant-derived odorants in insects, but their exact biochemistry and physiological functions remain unclear. RESULTS In this study, two paralogous antennae-enriched CCEs from Plutella xylostella (PxylCCE16a and 16c) were identified and functionally characterized. High-purity protein preparations of active recombinant PxylCCE16a and 16c have been obtained from Sf9 insect cells by Ni2+ affinity purification. Our results revealed that the purified recombinant PxylCCE016c is able to degrade two sex pheromone components Z9-14:Ac and Z11-16:Ac at 27.64 +/- 0.79% and 24.40 +/- 3.07%, respectively, while PxylCCE016a presented relatively lower activity. Additionally, a similar difference in activity was measured in plant-derived odorants. Furthermore, both CCEs displayed obvious preferences for the two sex pheromone components, especially on Z11-16:Ac (K-m values are in the range 7.82-45.06 mu mol L-1) which much lower than plant odorants (K-m values are in the range 1290-4030 mu mol L-1). Furthermore, the activity of the two newly identified CCEs is pH-dependent. The activity at pH 6.5 is obviously higher than that at pH 5.0. Interestingly, only PxylCCE016c can be inhibited by a common esterase inhibitor triphenyl phosphate (TPP) with LC50 of 1570 +/- 520 mu mol L-1. CONCLUSION PxylCCE16c plays a more essential role in odorant degradation than PxylCCE16a. Moreover, the current study provides novel potential pesticide targets for the notorious moth Plutella xylostella.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序