Carbamoyl phosphate synthetase subunit Cpa1 interacting with Dut1, controls development, arginine biosynthesis, and pathogenicity of Colletotrichum gloeosporioides

文献类型： 外文期刊

第一作者： Tan, Qingqun

作者： Tan, Qingqun;Yi, Tuyong;Zhao, Xuanzhu;Zhang, Junxiang;Tan, Qingqun;He, Haiyong

作者机构：

关键词： Glomerella cingulata; Virulence; Apple; Mitochondria; Anthracnose

期刊名称：FUNGAL BIOLOGY （ 影响因子：3.099； 五年影响因子：3.364 ）

ISSN： 1878-6146

年卷期： 2021 年 125 卷 3 期

页码：

收录情况： SCI

摘要： Carbamoyl phosphate synthetase is involved in arginine biosynthesis in many organisms. In this study, we investigate the biological function of Cpa1, a small subunit of carbamoyl phosphate synthetase of Colletotrichum gloeosporioides. The deletion of the CPA1 gene affected vegetative growth, arginine biosynthesis, and fungal pathogenicity. Genetic complementation with native CPA1 fully recovered all these defective phenotypes. We observed that Cpa1-RFP fusion protein is localized at the mitochondria, which is consistent with Cpa2, a large subunit of carbamoyl phosphate synthetase. We identified the proteins that interact with Cpa1 by using the two-hybrid screen approach, and we showed that Dut1 interacts with Cpa1 but without Cpa2 in vivo. Dut1 is dispensable for hyphal growth, appressorial formation, and fungal pathogenicity. Interestingly, the Dut1-Cpa1 complex is localized at the mitochondria. Further studies showed that Dut1 regulates Cpa1-Cpa2 interaction in response to arginine. In summary, our studies provide new insights into how Cpa1 interacts with its partner proteins to mediate arginine synthesis. (C) 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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